File:Zebrafish Embryonic Notochord Development The pacsin3 MO phenotype.jpg
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Figure 5. The pacsin3 MO phenotype. (A–D) Lateral notochord morphology in 5 ng control or pacsin3 MO-injected 24 hpf embryos treated with (B and D) or without (A and C) 10 mM b-aminoproprionitrile to disrupt the notochordal sheath. The relative angle of the normally chevron-shaped somites is indicated (purple). Anterior is left. Bar = 250 mm. (E–F) Thin section EM images of the trilaminar perinotochordal sheath in 5 ng control or pacsin3 MO-injected 24 hpf embryos. Arrows demarcate the boundary of the sheath. Bar = 0.5 mm. (G–J) Close-up dorsal views of the chordamesoderm at the 10-somite stage in 5 ng control (G and I) or pacsin3 (H and J) MO-injected embryos. Anterior is left. (I–J) Embryonic myoD mRNA localization by whole mount in situ. Bar = 250 mm. (K–L) Representative confocal sections of the lateral notochord region from BODIPY-Texas red labeled [88] live 24 hpf embryos after 5 ng control or pacsin3 MO injection. Bar = 50 mm. (M–N) Thin section EM micrographs of cross-sections through the notochord of 5 ng control or pacsin3 MO-injected 24 hpf embryos. Extra-notochord tissue is pseudocolored yellow, and vacuoles (V) and the perinotochordal sheath (arrows) are indicated. Bar = 10 mm. (O–R) Embryonic sonic hedgehog (shh) and patched1 (ptc1) mRNA localization by whole mount in situ in 5 ng control or pacsin3 MO-injected 24 hpf embryos. Bar = 250 m |
| Date | |
| Source | Edeling MA, Sanker S, Shima T, Umasankar PK, Ho¨ ning S, et al. (2009) Structural Requirements for PACSIN/Syndapin Operation during Zebrafish Embryonic Notochord Development. PLoS ONE 4(12): e8150. doi:10.1371/journal.pone.0008150 https://d-scholarship.pitt.edu/13299/1/Structural_Requirements.pdf |
| Author | James Keen, Thomas Jefferson University, United States of America |
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ß 2009 Edeling et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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| current | 10:12, 27 April 2024 | | 843 × 895 (831 KB) | Rasbak (talk | contribs) | {{Information |description=Figure 5. The pacsin3 MO phenotype. (A–D) Lateral notochord morphology in 5 ng control or pacsin3 MO-injected 24 hpf embryos treated with (B and D) or without (A and C) 10 mM b-aminoproprionitrile to disrupt the notochordal sheath. The relative angle of the normally chevron-shaped somites is indicated (purple). Anterior is left. Bar = 250 mm. (E–F) Thin section EM images of the trilaminar perinotochordal sheath in 5 ng control or pacsin3 MO-injected 24 hpf embryos.... |
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