File:Live-Imaging-of-Innate-Immune-Cell-Sensing-of-Transformed-Cells-in-Zebrafish-Larvae-Parallels-pbio.1000562.s003.ogv
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[edit]DescriptionLive-Imaging-of-Innate-Immune-Cell-Sensing-of-Transformed-Cells-in-Zebrafish-Larvae-Parallels-pbio.1000562.s003.ogv |
English: Supporting movies for Figure 1B. (A) shows several MPO:GFP+ neutrophils as they are recruited to a mitfa:V12RAS-mitfa:mCherry-expressing clone of melanocytes in the flank skin of a 5-dpf zebrafish larva (Figure 1Bi). The movie was taken at one frame every 90 s. Black pigmentation quenches much of the mCherry signal (red). The DIC channel is overlayed to show the outline of the V12RAS+ clone. The yellow arrowhead in the movie indicates a small fragment of a V12RAS+ melanocyte as it is engulfed by an MPO:GFP+ neutrophil; later this fragment is seen as a black phagosome inside the neutrophil cell body. (B) is the same sequence as in (A) but without the DIC channel. (C) is a control larva with melanoblasts expressing mCherry alone; in this example, several MPO:GFP+ cells come close to the mCherry-expressing cells but are not drawn to the clone. |
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Source | Video S1 from Feng Y, Santoriello C, Mione M, Hurlstone A, Martin P (2010). "Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound Inflammation". PLOS Biology. DOI:10.1371/journal.pbio.1000562. PMID 21179501. PMC: 3001901. | ||
Author | Feng Y, Santoriello C, Mione M, Hurlstone A, Martin P | ||
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current | 00:47, 31 October 2012 | 11 s, 1,535 × 512 (2.09 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Feng Y, Santoriello C, Mione M, Hurlstone A, Martin P |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Supporting movies for Figure 1B. (A) shows several MPO:GFP+ neutrophils as they are recruited to a mitfa:V12RAS-mitfa:mCherry-expressing clone of melanocytes in the flank skin of a 5-dpf zebrafish larva (Figure 1Bi). The movie was taken at one frame every 90 s. Black pigmentation quenches much of the mCherry signal (red). The DIC channel is overlayed to show the outline of the V12RAS+ clone. The yellow arrowhead in the movie indicates a small fragment of a V12RAS+ melanocyte as it is engulfed by an MPO:GFP+ neutrophil; later this fragment is seen as a black phagosome inside the neutrophil cell body. (B) is the same sequence as in (A) but without the DIC channel. (C) is a control larva with melanoblasts expressing mCherry alone; in this example, several MPO:GFP+ cells come close to the mCherry-expressing cells but are not drawn to the clone. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010-12 |
Categories:
- Molecular development
- Videos of disease models
- Innate immunity
- Leukocyte activation
- Videos of neoplasms of the digestive system
- Videos of skin cancers
- Immune response
- Dysplasias
- Animal models of disease
- Gene knockdown techniques
- Larvae
- Videos of melanocytes
- Videos of melanoma
- Confocal microscopic movies from Open Access Journals
- Videos of morpholines
- NADPH oxidase
- Proto-oncogene proteins p21
- Videos of wounds
- Videos of Zebrafish
- Zebrafish proteins
- MCherry