File:PhOTO-Zebrafish-A-Transgenic-Resource-for-In-Vivo-Lineage-Tracing-during-Development-and-pone.0032888.s008.ogv
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[edit]DescriptionPhOTO-Zebrafish-A-Transgenic-Resource-for-In-Vivo-Lineage-Tracing-during-Development-and-pone.0032888.s008.ogv |
English: Segmented Time-Lapse Data for PhOTO-M Membrane Dynamics Visualization During Late Gastrulation. This video shows segmented data for each frame of the time-lapse summarized in Figure 2 in the text. First, the photoconverted membranes were segmented in 3D. Then, using the segmented data, nuclei within the surrounding photoconverted membranes were identified and represented as colored surfaces. The nuclei were tracked starting with the final frame, moving backward in time. (A) Segmented and photoconverted memb-Dendra2 (red) as well as the segmented H2B-Cerulean channel (multi-colored surfaces) along with the bright field (grayscale) from the time-lapse video. Note that the developing head and optic primordia can be seen during the time-lapse. (B) Segmented and photoconverted memb-Dendra2 (red) as well as the segmented H2B-Cerulean (multi-colored surfaces) along with the merged animal pole images as seen in Video S3D. (C) Isolated zoomed images of the segmented nuclei and membranes during the time-lapse. Scale bars are 50µm. |
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Source | Video S4 from Dempsey W, Fraser S, Pantazis P (2012). "PhOTO Zebrafish: A Transgenic Resource for In Vivo Lineage Tracing during Development and Regeneration". PLOS ONE. DOI:10.1371/journal.pone.0032888. PMID 22431986. PMC: 3303793. | ||
Author | Dempsey W, Fraser S, Pantazis P | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:15, 30 October 2012 | 2.5 s, 1,972 × 1,476 (3.08 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Dempsey W, Fraser S, Pantazis P |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |